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Calcium mediates the light-induced decrease in maintained K+ current in Limulus ventral photoreceptors.

K Chinn, J Lisman

    The Journal of General Physiology
    |September 1, 1984
    PubMed
    Summary
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    Light exposure in Limulus photoreceptors triggers a decrease in potassium current (iK). This study demonstrates that intracellular calcium ions (Cai2+) mediate this light-induced reduction, acting as a key signaling molecule.

    Area of Science:

    • Neuroscience
    • Phototransduction
    • Cellular Physiology

    Background:

    • Light stimulation in Limulus ventral photoreceptors increases sodium conductance.
    • A concurrent, long-lasting reduction in voltage-dependent potassium current (iK) is observed.
    • The underlying mechanism for this iK reduction remains to be elucidated.

    Purpose of the Study:

    • To investigate the mechanism responsible for the light-induced decrease in voltage-dependent potassium current (iK).
    • To determine the role of intracellular free calcium (Cai2+) in mediating this light response.

    Main Methods:

    • Intracellular injection of calcium into Limulus ventral photoreceptors.
    • Measurement of voltage-dependent outward currents (iK).
    • Utilizing calcium buffer (EGTA) and pH buffer (MOPS) to assess the role of calcium and pH.

    Related Experiment Videos

  • Blocking voltage-dependent inward current (iin) with Ni2+ to isolate the effect on iK.

    • Main Results:

    • Intracellular calcium injection mimicked the effect of light by reducing the voltage-dependent outward current (iK).
    • Calcium-induced reduction of iK occurred independently of voltage-dependent inward current (iin) and involved a decrease in conductance.
    • The light-induced decrease in iK was blocked by the calcium buffer EGTA, but not by the pH buffer MOPS.
    • Intracellular free calcium (Cai2+) was shown to directly reduce iK.

    Conclusions:

    • Intracellular free calcium (Cai2+) is a critical mediator of the light-induced decrease in voltage-dependent potassium current (iK) in Limulus ventral photoreceptors.
    • These findings support the hypothesis that calcium acts as the internal transmitter for the photic regulation of iK.