Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human antisera detect a Plasmodium falciparum genomic clone encoding a nonapeptide repeat.

M Koenen, A Scherf, O Mercereau

    Nature
    |September 3, 1984
    PubMed
    Summary

    Researchers developed a new method to identify malaria parasite antigens. This approach uses genomic DNA fragments to find Plasmodium falciparum antigens, aiding vaccine development.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Association between exposure to pesticides and toxicity in humans: a review.

    European review for medical and pharmacological sciences·2025
    Same author

    Methanonatronarchaeum thermophilum gen. nov., sp. nov. and 'Candidatus Methanohalarchaeum thermophilum', extremely halo(natrono)philic methyl-reducing methanogens from hypersaline lakes comprising a new euryarchaeal class Methanonatronarchaeia classis nov.

    International journal of systematic and evolutionary microbiology·2018
    Same author

    Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2016
    Same author

    PKA and Apicomplexan Parasite Diseases.

    Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2016
    Same author

    Orientation relationship of eutectoid FeAl and FeAl<sub>2</sub>.

    Journal of applied crystallography·2016
    Same author

    Control of downy mildew (Pseudoperonospora cubensis) of greenhouse grown cucumbers with alternative biological agents.

    Communications in agricultural and applied biological sciences·2011

    Area of Science:

    • Malariology
    • Molecular Biology
    • Immunology

    Background:

    • Malaria, caused by Plasmodium falciparum, is a significant global health issue, particularly in tropical regions.
    • Development of an effective malaria vaccine requires the identification and characterization of parasite antigens.
    • Existing methods involve analyzing complementary DNA (cDNA) libraries expressed in Escherichia coli.

    Purpose of the Study:

    • To construct a novel expression bank using randomly generated fragments of Plasmodium falciparum genomic DNA.
    • To identify Plasmodium falciparum antigens reactive with human immune sera.
    • To characterize the antigenic determinants expressed by selected clones.

    Main Methods:

    • Construction of a Plasmodium falciparum genomic DNA expression bank in Escherichia coli.

    Related Experiment Videos

  • Screening of the expression bank using immunodetection with human African immune sera.
  • Analysis of the peptide sequences of reactive clones, focusing on repetitive units.

    • Main Results:

    • Several clones expressing Plasmodium falciparum antigens were identified.
    • One clone strongly reacted with human African immune sera.
    • This clone expressed an antigenic determinant containing degenerated repeats of a peptide nonamer.

    Conclusions:

    • Directly generating expression banks from genomic DNA is a viable strategy for identifying Plasmodium falciparum antigens.
    • The identified antigens, particularly those with repetitive peptide structures, are potential targets for malaria vaccine development.
    • This method complements existing cDNA-based approaches for parasite antigen discovery.