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Related Experiment Videos

Agarose: a possible universal gel exclusion agent.

A Polson, K J van der Merwe

    Preparative Biochemistry
    |January 1, 1984
    PubMed
    Summary
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    New granulated agarose gels for gel exclusion chromatography can now be prepared. This method allows for the purification of proteins across all molecular weights using a novel concentration technique.

    Area of Science:

    • Biochemistry
    • Chromatography

    Background:

    • Agarose gels are widely used in chromatography for separating molecules.
    • Preparing granulated agarose gels with a wide range of concentrations has been challenging.

    Purpose of the Study:

    • To develop a method for preparing granulated agarose gels suitable for gel exclusion chromatography of proteins of any molecular weight.
    • To enable the preparation of gels with concentrations ranging from low to 16%.

    Main Methods:

    • Utilizing a novel technique involving the displacement of agarose from solution using polyethylene glycol (Mr 6000).
    • Concentrating the displaced agarose polysaccharide into a viscous mass.
    • Diluting the concentrated polysaccharide with hot electrolyte solutions and allowing it to congeal.

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    Main Results:

    • Successfully prepared granulated agarose gels with concentrations up to 16%.
    • Demonstrated the suitability of these gels for gel exclusion chromatography of proteins across a broad molecular weight range.

    Conclusions:

    • The described method provides a versatile approach to creating granulated agarose gels for protein purification.
    • This advancement expands the utility of agarose gel chromatography for diverse protein separation applications.