Establishment of a human diploid cell strain

A new human diploid fibroblast-like cell strain has been established from dermal tissue of a 2 months old female embryo. This cell strain showed diploid karyotype and has a finite life span of 95-100 generations. No spontaneous transformation was observed during the whole life span in vitro. This cell strain is susceptible to SV40 infection and can be transformed easily by it.