Quantitative modification of immunohaemolytic sensitivity of bovine erythrocytes

Experimental work was carried out to study the effect of periodate and neuraminidase on the J-specific and non-J-specific immunohaemolysis of bovine erythrocytes. The method of quantitative immunohaemolysis was applied for the determination of immunohaemolytic sensitivity. Both the periodate and neuraminidase treatment, while increasing J-specific immunohaemolysis, resulted in non-J-specific haemolysis. Periodate treatment that followed a neuraminidase one, however, decreased the enhancement of J-specific immunohaemolysis and resulted in a significant increase of non-J-specific haemolysis. The maximum increase of non-J-specific haemolysis was found in erythrocytes highly sensitive to anti-J-serum.