Hepatitis B virus transcription in the infected liver

Hepatitis B virus (HBV) transcription was studied in the liver of an infected chimpanzee and compared with HBV transcription in heterologous systems. Besides the well characterized 2.3-kb surface antigen mRNA produced in most systems, a second major transcript was identified in the liver. This 3.8-kb transcript (+/- 300 bases) is slightly larger than the HBV genome and is probably involved both in core/e antigen synthesis and in HBV replication via reverse transcription. In addition, minor variants of the 2.3-kb surface antigen mRNA were characterized as probably being involved in the expression of HBsAg-related minor proteins. Finally, several potential transcription signals, identified on the HBV genome using heterologous expression systems, were found to be poorly active if at all in the infected liver, thereby stressing the importance of HBV transcription studies performed with liver material.