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Related Experiment Videos

Differentiation-inducing agents decrease cryptic prolactin receptors in cultured rat mammary tumor cells.

M E Costlow

    Experimental Cell Research
    |November 1, 1984
    PubMed
    Summary

    Cryptic prolactin receptors in mammary cells decrease with differentiation. Sodium butyrate and dimethyl sulfoxide (DMSO) reduced these receptors, inhibited cell growth, and promoted differentiation markers in rat mammary tumor cultures.

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    Area of Science:

    • Cell Biology
    • Endocrinology
    • Cancer Research

    Background:

    • Normal and neoplastic mammary cells possess cryptic prolactin receptors, largely unmasked by energy depletion.
    • These receptors differ from those in lactating mammary tissue, suggesting a role in prolactin-mediated growth.
    • The significance of cryptic prolactin receptors in mammary cell proliferation and differentiation remains to be elucidated.

    Purpose of the Study:

    • To investigate the effects of differentiation-inducing agents, dimethylsulfoxide (DMSO) and sodium butyrate, on cryptic prolactin receptors in mammary cells.
    • To determine if these agents influence mammary cell growth and differentiation markers.
    • To explore the potential role of cryptic prolactin receptors in mammary cell differentiation.

    Main Methods:

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  • Primary cultures of 7,12-dimethylbenzanthracene-induced rat mammary tumors were treated with varying concentrations of sodium butyrate and DMSO.
  • Prolactin receptor levels, specifically cryptic sites, were quantified.
  • Cell growth and morphological features indicative of differentiation (e.g., dome formation, lipid accumulation) were assessed.

    • Main Results:

    • Both sodium butyrate and DMSO significantly decreased cryptic prolactin receptor levels in a time- and dose-dependent manner.
    • These agents inhibited mammary tumor cell growth.
    • Sodium butyrate and DMSO treatment led to increased dome formation and lipid droplet accumulation, characteristic of differentiated mammary cells.

    Conclusions:

    • The loss of cryptic prolactin receptors correlates with the induction of differentiation in mammary cells.
    • Differentiation-inducing agents like sodium butyrate and DMSO reduce cryptic prolactin receptors and inhibit cell proliferation.
    • Cryptic prolactin receptors may play a crucial role in the acquisition of a differentiated mammary cell phenotype.