Preparation and analysis of reactive blue 2 bonded to silica via variable spacer groups

A variety of aminoalkylated microparticulate porous silicas have been prepared and used to displace the reactive chloride from reactive blue 2 thereby immobilizing the dye in a mild and predictable manner. Methods of analysis of immobilized reactive blue 2 are compared and a rapid new method is recommended which is clean and non-destructive. The biospecific elution of a dehydrogenase from an immobilized dye column is demonstrated.