Radioimmunofocus assay for detection and quantitation of human rotavirus

A radioimmunofocus assay suitable for quantitation of cell culture-adapted human rotavirus was developed. The method was reproducible, more sensitive than plaque assay, and useful to detect and quantify strains of rotavirus which do not produce plaques. Preliminary results also suggested that the technique will be a useful means of serotyping cell culture-adapted strains of the virus.