Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Angiotensin I-converting enzyme in amniotic fluid.

T Yasui, F Alhenc-Gelas, P Corvol

The Journal of Laboratory and Clinical Medicine

|November 1, 1984

Summary

Human amniotic fluid contains angiotensin I-converting enzyme (ACE). This study found ACE and other components of the renin-angiotensin system in amniotic fluid, suggesting its presence during pregnancy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Tissue kallikrein is required for the cardioprotective effect of cyclosporin A in myocardial ischemia in the mouse.

Biochemical pharmacology·2015

Same author

Pathophysiology of genetic deficiency in tissue kallikrein activity in mouse and man.

Thrombosis and haemostasis·2013

Same author

Cardioprotection and kallikrein-kinin system in acute myocardial ischaemia in mice.

Clinical and experimental pharmacology & physiology·2008

Same author

Murine models of myocardial and limb ischemia: diagnostic end-points and relevance to clinical problems.

Vascular pharmacology·2006

Same author

[Gene transfers of kallikrein, bradykinin receptor B2 and a mutated B2 receptor stimulate neoangiogenesis in peripheral ischemia].

Archives des maladies du coeur et des vaisseaux·2005

Same author

Role of tissue kallikrein in the cardioprotective effects of ischemic and pharmacological preconditioning in myocardial ischemia.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2005

Area of Science:

Biochemistry
Reproductive Biology
Enzymology

Background:

Angiotensin I-converting enzyme (ACE) plays a crucial role in the renin-angiotensin system.
The presence and function of ACE in human amniotic fluid are not well-established.

Purpose of the Study:

To characterize the antigenic and enzymatic properties of ACE in human amniotic fluid.
To investigate the presence of other components of the renin-angiotensin system in amniotic fluid.

Main Methods:

Radioimmunoassay was used to quantify ACE antigen levels.
Enzymatic activity was measured using Hip-His-Leu as a substrate.
Inhibition studies with captopril and antibodies were performed.

Main Results:

ACE was detected in soluble form in human amniotic fluid (mean level 136 +/- 83 ng/ml).
Amniotic fluid ACE showed complete cross-reactivity with antibodies against lung ACE.
Significant correlation was found between ACE antigen levels and activity.
Angiotensinase, kininase, renin, and angiotensinogen were also identified in amniotic fluid.

Conclusions:

A complete, though not fully activated, renin-angiotensin system exists in human amniotic fluid.
ACE in amniotic fluid shares antigenic and enzymatic properties with ACE from other human tissues.
These findings suggest a potential role for the renin-angiotensin system in the amniotic fluid environment during pregnancy.

Related Experiment Videos