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Analysis of the Sendai virus M gene and protein
Abstract:
The nucleotide sequence of the Sendai virus M (matrix or membrane) gene region was determined from cloned genomic DNA, and the limits of the M mRNA were determined by S1 nuclease mapping. The M mRNA is 1,173 nucleotides long and contains a single long open reading frame coding for a protein of 348 amino acids. The amino acid sequences of the N- and C-terminal peptides of the M protein were obtained by mass spectrometric analysis and correspond to those predicted from the open reading frame, with the N terminus modified in vivo by cleavage of the initiating methionine and acetylation of the following amino acid. The amphiphilic nature of the M protein structure is discussed.
Insights
Researchers sequenced the Sendai virus M gene, revealing a 1,173-nucleotide mRNA encoding a 348-amino acid matrix protein. Mass spectrometry confirmed the protein sequence and N-terminal modifications.
Area of Science:
- Virology
- Molecular Biology
- Protein Chemistry
Background:
- Sendai virus is a paramyxovirus crucial for studying viral gene expression.
- The matrix (M) protein plays a key role in viral assembly and structure.
Purpose of the Study:
- To determine the nucleotide sequence of the Sendai virus M gene.
- To characterize the M protein's primary structure and modifications.
Main Methods:
- Cloned genomic DNA sequencing.
- S1 nuclease mapping to define mRNA.
- Mass spectrometric analysis of protein peptides.
Main Results:
- The M gene region sequence was determined.
- M mRNA is 1,173 nucleotides, encoding a 348-amino acid protein.
- N-terminal methionine cleavage and acetylation were observed in vivo.
Conclusions:
- The complete M gene sequence provides a basis for understanding M protein function.
- In vivo modifications affect the M protein's N terminus.
- The M protein exhibits an amphiphilic structure.