Alternative splicing in the 5' moiety of the H-2Kd gene transcript

The H-2Kd gene, which encodes a mouse major transplantation antigen, was transfected into L TK- mouse fibroblasts. Two transcripts of the gene were detected by S1 nuclease mapping analysis. They correspond to two previously characterized cDNA clones isolated from DBA/2 mouse liver RNA, leading to the conclusion that the H-2Kd gene gives rise to two distinct transcripts through an alternate use of splicing sites. The non-canonical RNA potentially encodes a so far undescribed H-2Kd-like molecule. It is present in all tissues tested (liver, spleen, thymus, kidney) albeit in lower amounts (approximately 10-fold less) than the canonical RNA coding for H-2Kd.