Jove
Visualize
Contact Us

Related Experiment Videos

A cryptogram for recording rotavirus strains: the Rotacode.

R B Moosai, R Alcock, C R Madeley

    The Journal of Hygiene
    |October 1, 1984
    PubMed
    Summary

    A new coding system, Rotacode, was developed to classify rotavirus strains based on their RNA genome patterns. This method reliably differentiates diverse wild rotavirus strains, revealing significant electrophoretic variability.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    COVID-19 lung disease is a pulmonary vasculopathy.

    Clinical radiology·2024
    Same author

    Determining the haemodynamic significance of arterial stenosis: the relationship between CT angiography, computational fluid dynamics, and non-invasive fractional flow reserve.

    Clinical radiology·2016
    Same author

    Cardiology Symposium 2013: Therapeutic and Technological Advances.

    The journal of the Royal College of Physicians of Edinburgh·2014
    Same author

    Antiphospholipid syndrome and rheumatic fever: a case spanning three decades of changing concepts and common immunological mechanisms.

    Lupus·2011
    Same author

    Observations on injuries of joints, and their treatment.

    Medico-chirurgical transactions·2010
    Same author

    An Address on Health.

    British medical journal·2010
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • Rotaviruses possess an 11-segment RNA genome.
    • Genome segmentation allows for classification via polyacrylamide gel electrophoresis (PAGE).
    • PAGE patterns can vary between rotavirus strains.

    Purpose of the Study:

    • To develop a standardized method for classifying rotavirus strains based on their electrophoretic genome patterns.
    • To introduce a shorthand coding system for rotavirus strain characterization.

    Main Methods:

    • Polyacrylamide gel electrophoresis (PAGE) was used to separate rotavirus RNA genome segments.
    • A cryptogram, termed Rotacode, was developed based on the relative migration distances of PAGE bands within size classes.
    • Rotacode was applied to analyze 189 rotavirus strains collected over three years.

    Main Results:

    • Rotacode provides a reliable and reproducible method for coding rotavirus electrophoretic patterns.
    • The system successfully differentiated 13 distinct electrophoretic types (electrophoretypes) among 189 analyzed strains.
    • Identical codes were consistently obtained across repeated experiments and by different observers.

    Conclusions:

    • Rotacode is an effective tool for documenting and grouping rotavirus strains with similar electrophoretic profiles.
    • The study highlights the significant electrophoretic variability present in wild rotavirus populations.
    • The proposed Rotacode system offers a valuable shorthand for rotavirus strain characterization in research and diagnostics.

    Related Experiment Videos