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Interactions between herpes simplex virus DNA-binding proteins.

P J Vaughan, L M Banks, D J Purifoy

    The Journal of General Virology
    |November 1, 1984
    PubMed
    Summary
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    Monoclonal antibodies reveal interactions between herpes simplex virus (HSV) proteins. These findings suggest HSV DNA-binding proteins may form a complex during viral DNA replication.

    Area of Science:

    • Virology
    • Molecular Biology
    • Immunology

    Background:

    • Herpes simplex virus (HSV) infection involves complex viral protein interactions.
    • Understanding these interactions is crucial for elucidating viral replication mechanisms.

    Purpose of the Study:

    • To investigate protein-protein interactions among HSV-induced proteins.
    • To explore the potential assembly of a viral DNA replication complex.

    Main Methods:

    • Utilized monoclonal antibodies specific to HSV-induced DNA-binding proteins.
    • Employed immunoadsorbent chromatography and affinity chromatography techniques.
    • Purified and identified interacting polypeptides from HSV-infected cells.

    Main Results:

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    • Monoclonal antibody against alkaline nuclease co-purified the major DNA-binding protein.
    • Monoclonal antibody against the major DNA-binding protein eluted DNA polymerase and alkaline nuclease.
    • Similar interactions were observed with antibodies against other HSV-2 DNA-binding proteins.

    Conclusions:

    • The results strongly suggest that HSV-induced DNA-binding proteins, including alkaline nuclease and DNA polymerase, interact with each other.
    • These interactions support the hypothesis that these proteins form a functional HSV DNA replication complex.