[Covalent derivatives of aminoacyl-tRNA-synthetases with substrates]

The possibility of participation of covalent enzyme substrate's derivatives in reactions catalyzed by aminoacyl-tRNA synthetases is discussed in connection with general principles of enzyme catalysis. Tryptophanylated and pyrophosphorylated forms of beef pancreas tryptophanyl-tRNA synthetase were identified, isolated and characterized and the expected role of these derivatives in the enzyme function is discussed.