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Related Experiment Videos

src- and fps-containing avian sarcoma viruses transform chicken erythroid cells.

P Kahn, B Adkins, H Beug

    Proceedings of the National Academy of Sciences of the United States of America
    |November 1, 1984
    PubMed
    Summary

    Several oncogenic avian sarcoma viruses, including Rous sarcoma virus (src) and Fujinami sarcoma virus (fps), can transform avian erythroid cells. These findings highlight the oncogenic potential of the src gene family beyond erbB.

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    Area of Science:

    • Oncology
    • Virology
    • Cell Biology

    Background:

    • Avian sarcoma viruses (ASVs) are known to carry oncogenes that can induce tumors.
    • Erythroid cell differentiation is a tightly regulated process.
    • The role of specific oncogenes in erythroid cell transformation and differentiation is not fully understood.

    Purpose of the Study:

    • To investigate the ability of different oncogene-transducing ASV strains (src, fps) to transform avian erythroid cells.
    • To compare the properties of src- and fps-transformed erythroblasts with those transformed by avian erythroblastosis virus (AEV).
    • To determine if other members of the src gene family, besides erbB, can induce erythroid cell differentiation.

    Main Methods:

    • Transformation of avian erythroid cells in vitro and in vivo using ASV strains (src, fps, PRCII).

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  • Culture and characterization of transformed erythroblasts under standard and special conditions.
  • Use of temperature-sensitive mutants of src and fps oncogenes.
  • Induction of terminal differentiation by shifting temperature in the presence of an erythropoietin-like factor.

    • Main Results:

    • src and fps oncogenes transform avian erythroid cells, leading to erythroblast proliferation with specific growth requirements and differentiation tendencies.
    • Transformed erythroblasts exhibit distinct growth and differentiation properties compared to those transformed by AEV-H (erbB) and AEV-ES4 (erbA, erbB).
    • Erythroid cells transformed by temperature-sensitive src or fps mutants undergo terminal differentiation into erythrocytes upon temperature shift.

    Conclusions:

    • Multiple members of the src gene family, beyond erbB, possess the capacity to transform erythroid cells.
    • The src and fps oncogenes play a significant role in the aberrant proliferation and differentiation of avian erythroid cells.
    • These findings contribute to understanding the oncogenic mechanisms of ASVs in erythroid lineage.