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Updated: Jun 25, 2026

A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis
Published on: August 12, 2010
A single troponin T gene regulated by different programs in cardiac and skeletal muscle development
Abstract:
A cloned complementary DNA derived from a messenger RNA transiently present at low abundance levels in early chick embryonic skeletal muscle hybridizes to a messenger RNA present at high abundance levels in cardiac muscle. Genomic DNA hybridization and nucleotide sequence identity of complementary DNA's from both heart and skeletal muscle demonstrate that the messenger RNA's from both sources are encoded by the same gene. The encoded polypeptide is a troponin T sequence which is probably a cardiac isoform. This single copy troponin T isogene is governed by different regulatory programs in heart and skeletal muscle differentiation.
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