Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Bacteriophages01:26

DNA Bacteriophages

1.1K
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
1.1K
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

1.9K
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
1.9K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

11.3K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
11.3K
Chromatin Packaging01:32

Chromatin Packaging

19.8K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
19.8K
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

78.3K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
78.3K
DNA Replication02:40

DNA Replication

60.7K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
60.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic and functional analysis of Vibrio phage SIO-2 reveals novel insights into ecology and evolution of marine siphoviruses.

Environmental microbiology·2012
Same author

Jumbo bacteriophages.

Current topics in microbiology and immunology·2009
Same author

Crosslinking in viral capsids via tiling theory.

Journal of theoretical biology·2005
Same author

Where are the pseudogenes in bacterial genomes?

Trends in microbiology·2002
Same author

Muscle strength in knee varus and valgus.

Medicine and science in sports and exercise·2001
Same author

Virus maturation involving large subunit rotations and local refolding.

Science (New York, N.Y.)·2001
Same journal

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
See all related articles

Related Experiment Video

Updated: Feb 17, 2026

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
08:51

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide

Published on: June 23, 2016

11.3K

Length determination in bacteriophage lambda tails.

I Katsura, R W Hendrix

    Cell
    |December 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Researchers identified bacteriophage lambda mutants with deletions in gene H, affecting tail protein length. These findings suggest the H protein acts as a ruler to determine phage tail length during assembly.

    More Related Videos

    Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
    08:31

    Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

    Published on: May 26, 2013

    11.5K
    Following Cell-fate in E. coli After Infection by Phage Lambda
    06:10

    Following Cell-fate in E. coli After Infection by Phage Lambda

    Published on: October 14, 2011

    24.2K

    Related Experiment Videos

    Last Updated: Feb 17, 2026

    Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
    08:51

    Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide

    Published on: June 23, 2016

    11.3K
    Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
    08:31

    Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

    Published on: May 26, 2013

    11.5K
    Following Cell-fate in E. coli After Infection by Phage Lambda
    06:10

    Following Cell-fate in E. coli After Infection by Phage Lambda

    Published on: October 14, 2011

    24.2K

    Area of Science:

    • Molecular Biology
    • Virology
    • Genetics

    Background:

    • Bacteriophage lambda is a well-studied virus with a complex tail structure.
    • The tail structure is essential for phage infection and DNA injection.
    • Gene H codes for a minor tail protein, but its precise function in tail assembly was unclear.

    Purpose of the Study:

    • To investigate the role of bacteriophage lambda's gene H in tail assembly and length determination.
    • To characterize mutants with deletions in gene H and their impact on phage particle structure.

    Main Methods:

    • Isolation and characterization of viable bacteriophage lambda mutants with in-frame deletions in gene H.
    • Analysis of gene H protein products and their effect on phage particle assembly.
    • Correlation of deletion size with resulting tail length deficiency.

    Main Results:

    • Mutants produced smaller but active gene H protein products.
    • Phage particles assembled with these mutants exhibited shorter tails.
    • Tail length deficiency directly corresponded to the calculated shortening of the gene H protein.

    Conclusions:

    • The H protein of bacteriophage lambda dictates the length of the phage tail.
    • The H protein likely functions as a ruler or template during tail assembly.
    • This provides a model for understanding structural protein function in viral assembly.