Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

42.4K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
42.4K
Conjugation01:19

Conjugation

3.0K
Conjugation is a form of horizontal gene transfer that primarily occurs in bacteria and some archaea, promoting genetic diversity and adaptation. Bacteria can acquire resistance genes through conjugative plasmids, allowing them to survive antibiotic treatments that would otherwise be lethal. This process involves direct contact between cells through specialized structures such as the sex pilus and is mediated by conjugative plasmids, including the F (fertility) factor.Conjugation requires...
3.0K
Mechanism of Conjugation01:19

Mechanism of Conjugation

1.6K
Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
1.6K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

836
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
836
Microbiota of the Large Intestine01:27

Microbiota of the Large Intestine

98
The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...
98
Colonisation of Pathogens01:25

Colonisation of Pathogens

77
Pathogen colonization of host tissues is a critical step in the development of infectious diseases. Various pathogenic microorganisms, including bacteria, fungi, viruses, and protozoa, have evolved complex strategies to attach to, invade, and persist within host environments. These mechanisms enable pathogens to establish infections, evade immune responses, and resist antimicrobial treatments.Attachment to Host CellsIn bacteria, colonization typically begins with adherence to host epithelial...
77

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A study of antibiotic-producing bacteria from the soil.

Antibiotics & chemotherapy (Northfield, Ill.)·2014
Same author

Drosophila social clustering is disrupted by anesthetics and in narrow abdomen ion channel mutants.

Genes, brain, and behavior·2013
Same author

Biological inactivity of zeta-carotene.

Archives of biochemistry·2010
Same author

In vivo genetics of anaesthetic action.

British journal of anaesthesia·2002
Same author

Volatile general anesthetics reveal a neurobiological role for the white and brown genes of Drosophila melanogaster.

Journal of neurobiology·2001
Same author

Tn7: smarter than we thought.

Nature reviews. Molecular cell biology·2001
Same journal

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
See all related articles

Related Experiment Video

Updated: May 4, 2026

Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
11:12

Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

6.8K

E. coli integration host factor binds to specific sites in DNA.

N L Craig, H A Nash

    Cell
    |December 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Integration host factor (IHF) from E. coli binds specifically to DNA, influencing phage lambda recombination and gene expression. Its interaction sites on DNA reveal a conserved sequence critical for this binding.

    More Related Videos

    A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model
    08:14

    A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

    Published on: February 28, 2018

    8.5K
    Colonization with Murine pks+ Escherichia coli under Non-Inflammatory Conditions
    07:20

    Colonization with Murine pks+ Escherichia coli under Non-Inflammatory Conditions

    Published on: March 10, 2026

    140

    Related Experiment Videos

    Last Updated: May 4, 2026

    Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
    11:12

    Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

    Published on: September 11, 2017

    6.8K
    A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model
    08:14

    A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

    Published on: February 28, 2018

    8.5K
    Colonization with Murine pks+ Escherichia coli under Non-Inflammatory Conditions
    07:20

    Colonization with Murine pks+ Escherichia coli under Non-Inflammatory Conditions

    Published on: March 10, 2026

    140

    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Integration host factor (IHF) is a protein in E. coli involved in phage lambda site-specific recombination.
    • IHF also plays a role in regulating the expression of both phage and bacterial genes.

    Purpose of the Study:

    • To investigate the specific DNA-binding interactions of E. coli integration host factor (IHF).
    • To identify the DNA sequences recognized by IHF and their functional relevance.

    Main Methods:

    • Utilized protection from nuclease and chemical attack assays to study IHF-DNA interactions.
    • Analyzed DNA binding sites of IHF within the phage lambda attP recombination site and in non-att DNA regions.

    Main Results:

    • Demonstrated that IHF is a specific DNA-binding protein.
    • Identified three distinct segments within the attP site that interact with IHF.
    • Discovered specific IHF binding sites in non-att DNA, often adjacent to genes affected in IHF mutants.

    Conclusions:

    • IHF exhibits specific DNA binding, crucial for its roles in recombination and gene regulation.
    • A conserved DNA sequence motif (T.PyAA...PuTTGaT.A.PuTT...PyAACtA) is proposed as the critical determinant for IHF-DNA interaction.