Genomic analysis of avian and feline ureaplasmas by restriction endonucleases

Genomic relationship among the strains of avian and feline ureaplasmas was determined by restriction analysis. Chromosomal DNAs extracted from the two avian and four feline ureaplasma strains resolved in 1.0% agarose gel electrophoresis following digestion with the restriction enzymes, PstI, BamHI, HindIII, EcoRI, SalI and HpaII. Relationship among the strains was determined from base substitution frequency obtained by comparing the restriction patterns. The guanine plus cytosine (G + C) contents of the representative strains of avian and feline ureaplasmas were estimated by high-performance liquid chromatography to be 27.6 and 27.1 mole %, respectively. Restriction patterns of the avian and feline ureaplasmas were distinct from those of the human and bovine strains, but they were relatively similar within a serotype.