Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Yeast regulatory gene PPR1. I. Nucleotide sequence, restriction map and codon usage.

B Kammerer, A Guyonvarch, J C Hubert

    Journal of Molecular Biology
    |December 5, 1984
    PubMed
    Summary

    The Saccharomyces cerevisiae PPR1 gene

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Measure of asymmetrical transcription of the yeast OMP decarboxylase gene expressed in yeast or in E. coli.

    Current genetics·2013
    Same author

    Specific and non-specific immunity of piglets from sows fed diets containing specific fatty acids in field conditions.

    Journal of animal physiology and animal nutrition·2012
    Same author

    Effect of arsenite on swimming motility delays surface colonization in Herminiimonas arsenicoxydans.

    Microbiology (Reading, England)·2010
    Same author

    In vitro metabolite identification of ML3403, a 4-pyridinylimidazole-type p38 MAP kinase inhibitor by LC-Qq-TOF-MS and LC-SPE-cryo-NMR/MS.

    Xenobiotica; the fate of foreign compounds in biological systems·2007
    Same author

    Postprandial kinetics of some biotic and abiotic characteristics of the gastric ecosystem of horses fed a pelleted concentrate meal.

    Journal of animal science·2007
    Same author

    Corynebacterium glutamicum superoxide dismutase is a manganese-strict non-cambialistic enzyme in vitro.

    Microbiological research·2006

    Area of Science:

    • Molecular Biology
    • Yeast Genetics
    • Gene Regulation

    Background:

    • The PPR1 gene in Saccharomyces cerevisiae regulates the transcription of URA1 and URA3.
    • Understanding regulatory gene structure is crucial for deciphering gene expression control.

    Purpose of the Study:

    • To determine the primary structure of the eukaryotic regulatory PPR1 gene and its flanking regions.
    • To analyze the codon usage pattern within the PPR1 gene.

    Main Methods:

    • Dideoxynucleotide chain termination method was employed for DNA sequencing.
    • Bioinformatic analysis was used to examine the open reading frame and codon usage.

    Main Results:

    • An open reading frame of 2712 nucleotides (encoding 904 amino acids) was identified.
    • The 3' untranslated mRNA region contains typical yeast termination and polyadenylation signals.
    • Codon usage within the PPR1 gene was found to be random.

    Conclusions:

    • The primary structure of the PPR1 gene has been elucidated.
    • The random codon usage pattern is discussed in the context of protein abundance and compared to other yeast and E. coli genes.

    Related Experiment Videos