Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse c-mos oncogene activation is prevented by upstream sequences.

T G Wood, M L McGeady, B M Baroudy

    Proceedings of the National Academy of Sciences of the United States of America
    |December 1, 1984
    PubMed
    Summary

    A newly identified upstream mouse sequence (UMS) region prevents the activation of the c-mos oncogene by a 3' retroviral long terminal repeat (LTR). This finding explains the low transformation frequency and lack of c-mos transcripts in normal cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Black Hole Spectroscopy and Tests of General Relativity with GW250114.

    Physical review letters·2026
    Same author

    GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

    Physical review letters·2025
    Same author

    DIFFERENTIATION OF RAT LENS EPITHELIAL CELLS IN TISSUE CULTURE (III) FUNCTIONS IN VITRO OF A TRANSFORMED RAT LENS EPITHELIAL CELL LINE.

    Development, growth & differentiation·2023
    Same author

    Search for Subsolar-Mass Binaries in the First Half of Advanced LIGO's and Advanced Virgo's Third Observing Run.

    Physical review letters·2022
    Same author

    Acoustic and vibration isolation for a gravity gradiometer.

    The Review of scientific instruments·2022
    Same author

    Constraints on Cosmic Strings Using Data from the Third Advanced LIGO-Virgo Observing Run.

    Physical review letters·2021

    Area of Science:

    • Molecular biology
    • Oncogene research
    • Gene regulation

    Background:

    • The mouse c-mos oncogene can be activated by retroviral long terminal repeat (LTR) insertion.
    • Activation is efficient when the LTR is 5' to the coding region, but inefficient when 3'.

    Purpose of the Study:

    • To identify the DNA sequences responsible for the differential activation of the c-mos oncogene by 3' LTR insertion.
    • To elucidate the mechanism by which these sequences regulate c-mos expression.

    Main Methods:

    • Analysis of transformed cell lines with deletions in sequences preceding c-mos.
    • Determination of transforming potential of deletion mutants.
    • Nucleotide sequence analysis of identified regions.
    • Functional assays involving insertion of identified regions 5' to the v-mos coding region.

    Related Experiment Videos

    Main Results:

    • A 1 kb region, termed upstream mouse sequence (UMS), located 0.8-1.8 kb upstream of the c-mos coding region, was identified.
    • UMS acts as a cis-acting locus preventing c-mos activation by a 3' LTR.
    • UMS inhibits 3' LTR enhancement of v-mos transforming activity in a position-dependent manner.

    Conclusions:

    • UMS functions to regulate c-mos proto-oncogene expression.
    • The UMS region likely explains the absence of detectable c-mos transcripts in normal mouse cells.