Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bicuculline epileptogenesis in the rat.

A M Campbell, O Holmes

    Brain Research
    |December 10, 1984
    PubMed
    Summary

    Bicuculline application in rat brains revealed an optimal depth of 0.65 mm for inducing epileptiform discharges. This finding suggests a common mechanism for bicuculline and penicillin in causing seizures by blocking inhibitory neurotransmission.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Social-pair judgment bias testing in slow-growing broiler chickens raised in low- or high-complexity environments.

    Scientific reports·2023
    Same author

    Comparison of actionable events detected in cancer genomes by whole-genome sequencing, in silico whole-exome and mutation panels.

    ESMO open·2022
    Same author

    Impact of environmental complexity and stocking density on affective states of rainbow trout (Oncorhynchus mykiss).

    Animal cognition·2022
    Same author

    Environmental complexity positively impacts affective states of broiler chickens.

    Scientific reports·2021
    Same author

    Whole-genome sequencing reveals clinically relevant insights into the aetiology of familial breast cancers.

    Annals of oncology : official journal of the European Society for Medical Oncology·2019
    Same author

    Sci-Fri AM: YIS-04: Scatter correction of cone beam optical computed tomography for polymer gel dosimetry.

    Medical physics·2017

    Area of Science:

    • Neuroscience
    • Pharmacology
    • Epileptology

    Background:

    • GABAergic inhibition is crucial for regulating neuronal excitability in the cerebral cortex.
    • Understanding the precise localization of drug effects is key to elucidating epileptogenesis.

    Purpose of the Study:

    • To determine the optimal depth for bicuculline application to induce focal interictal epileptiform discharges (FIEDs) in the rat somatosensory cortex.
    • To investigate the potential common mechanisms underlying the epileptogenic actions of bicuculline and penicillin.

    Main Methods:

    • Electrophoretic application of bicuculline via microelectrode in urethane-anesthetized rats.
    • Recording of spontaneous FIEDs using a nearby microelectrode at varying cortical depths.
    • Analysis of the delay to onset, frequency, and voltage excursion of FIEDs in relation to drug application depth.

    Main Results:

    • The shortest delay to FIED onset and the greatest subsequent frequency and voltage excursion occurred when bicuculline was applied at 0.65 mm below the pial surface.
    • The depth-dependent sensitivity profile for bicuculline closely mirrored that of penicillin.
    • Low concentrations of both bicuculline and penicillin are known to block GABAergic inhibition.

    Conclusions:

    • The findings suggest that the epileptogenic effects of bicuculline and penicillin may share a common mechanism, likely the blockade of GABAergic inhibitory synaptic transmission.
    • The optimal depth for inducing seizures points to specific cortical layers or neuronal populations being particularly sensitive to GABAergic blockade.

    Related Experiment Videos