Autoradiographic distribution of nicotine receptors in rat brain

The autoradiographic visualisation of 90%-specific tritiated nicotine binding to slide-mounted sections of rat brain is reported. Tritiated nicotine bound with high affinity (nanomolar Kd) and was selectively displaced by nicotinic agonists (e.g. L-nicotine approximately ACh greater than D-nicotine). The strikingly discrete distribution pattern obtained deviates from that of alpha-bungarotoxin, and suggests several possible roles for nicotinic transmission in the brain.