Phenotype characteristics of human B cells studied by Epstein-Barr virus infection. II. C3 receptor switching during human B cell differentiation
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Our experiments showed that two C3 receptors (C3-R) appeared on membrane surfaces in a distinct sequence during the differentiation of human B cells. The experimental results supporting this conclusion were (1) the lymphoblastoid cells established from immature B cells expressed more EACm-R (C3bi and C3d receptors) than EACh-R (C3b receptor), (2) the lymphoblastoid cells originated from mature B cells showed more EACh-R than EACm-R, (3) the above characteristic expression of C3-R was demonstrated on every clone isolated in soft agar from cells in B cell lineage at various differentiation stages, indicating that a cell line consists of homogeneous population in terms of C3-R expression. From these results, it was concluded that C3-R switched from EACm-R to EACh-R during B cell differentiation.
Our experiments showed that two C3 receptors (C3-R) appeared on membrane surfaces in a distinct sequence during the differentiation of human B cells. The experimental results supporting this conclusion were (1) the lymphoblastoid cells established from immature B cells expressed more EACm-R (C3bi and C3d receptors) than EACh-R (C3b receptor), (2) the lymphoblastoid cells originated from mature B cells showed more EACh-R than EACm-R, (3) the above characteristic expression of C3-R was demonstrated on every clone isolated in soft agar from cells in B cell lineage at various differentiation stages, indicating that a cell line consists of homogeneous population in terms of C3-R expression. From these results, it was concluded that C3-R switched from EACm-R to EACh-R during B cell differentiation.