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Related Experiment Videos

Dimethyl sulfoxide decreases specific EGF binding.

H S Earp, Q Lin, J Blaisdell

    Journal of Cellular Biochemistry
    |January 1, 1984
    PubMed
    Summary
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    Dimethyl sulfoxide (DMSO) alters the binding affinity of the hepatic epidermal growth factor (EGF) receptor. This solvent-induced change in receptor conformation reversibly reduces EGF binding without requiring receptor autophosphorylation.

    Area of Science:

    • Cell biology
    • Biochemistry
    • Pharmacology

    Background:

    • The epidermal growth factor (EGF) receptor plays a crucial role in cellular signaling.
    • Dimethyl sulfoxide (DMSO) is a common solvent with known biological effects.
    • Understanding how solvents interact with cell surface receptors is important for experimental design.

    Purpose of the Study:

    • To investigate the effect of Dimethyl sulfoxide (DMSO) on the binding of epidermal growth factor (EGF) to its receptor.
    • To determine the mechanism by which DMSO influences EGF receptor activity.

    Main Methods:

    • Primary rat hepatocytes and purified rat liver plasma membranes were used.
    • Cells and membranes were treated with varying concentrations of DMSO.
    • 125I-EGF binding assays and Scatchard analysis were performed.

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  • Tyrosine kinase inhibition was employed to assess the role of autophosphorylation.

    • Main Results:

    • DMSO (1-10%) significantly reduced specific 125I-EGF binding to hepatocytes in a dose-dependent manner.
    • Maximum reduction in binding (40-60%) occurred at 5-7.5% DMSO and was reversible.
    • Scatchard analysis indicated that DMSO alters EGF receptor affinity, not the number of receptors.
    • The inhibitory effect of DMSO on EGF binding was observed even without ATP or in the presence of a tyrosine kinase inhibitor.

    Conclusions:

    • DMSO induces a conformational change in the hepatic EGF receptor that reversibly reduces its affinity for EGF.
    • This effect is independent of EGF receptor autophosphorylation.
    • DMSO's interaction with the EGF receptor highlights the importance of solvent choice in biological experiments.