Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mu-receptor binding in physiological media: comparison with isolated tissue data.

J A Carroll, L Miller, J S Shaw

    Neuropeptides
    |December 1, 1984
    PubMed
    Summary

    Mu-receptor affinities for opioid drugs were measured. Receptor binding assays in specific buffer conditions closely matched isolated tissue data, unlike other methods.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effects of oral meloxicam on physiological and behavioral outcomes of weaned calves following band castration.

    Translational animal science·2025
    Same author

    The Effect of Baseline Performance and Age on Cognitive Training Improvements in Older Adults: A Qualitative Review.

    The journal of prevention of Alzheimer's disease·2020
    Same author

    Use of a novel oleaginous microorganism as a potential source of lipids for weanling pigs<sup></sup>.

    Translational animal science·2020
    Same author

    Prion protein N1 cleavage peptides stimulate microglial interaction with surrounding cells.

    Scientific reports·2020
    Same author

    Response to adrenocorticotropic hormone or corticotrophin-releasing hormone and vasopressin in lactating cows fed an immunomodulatory supplement under thermoneutral or acute heat stress conditions.

    Journal of dairy science·2020
    Same author

    Pre-weaning plane of nutrition and Mannheimia haemolytica dose influence inflammatory responses to a bovine herpesvirus-1 and Mannheimia haemolytica challenge in post-weaning Holstein calves.

    Journal of dairy science·2019

    Area of Science:

    • Pharmacology
    • Neuroscience
    • Biochemistry

    Background:

    • Mu-opioid receptors are key targets for pain management.
    • Accurate determination of drug affinities is crucial for drug development.
    • Previous methods for measuring mu-opioid receptor affinities showed inconsistencies.

    Purpose of the Study:

    • To determine mu-receptor affinities for various opioid drugs.
    • To compare different techniques for measuring these affinities.
    • To identify reliable methods for assessing opioid drug interactions with mu-receptors.

    Main Methods:

    • Utilized isolated tissue preparations (rat vas deferens).
    • Employed receptor binding assays, including displacement of radioligands ([3H]-GLYOL and [3H]-naloxone).

    Related Experiment Videos

  • Varied buffer compositions (Tris vs. Krebs/HEPES with GTP analogue) for binding assays.

    • Main Results:

    • Isolated tissue data showed poor correlation with [3H]-GLYOL displacement in Tris buffer.
    • Isolated tissue data closely correlated with [3H]-naloxone displacement in Krebs/HEPES buffer with a GTP analogue.
    • This suggests specific buffer conditions are critical for accurate receptor binding measurements.

    Conclusions:

    • The choice of buffer and inclusion of GTP analogues significantly impact mu-opioid receptor binding assay results.
    • Receptor binding assays using [3H]-naloxone in Krebs/HEPES buffer with GTP analogue provide reliable affinity data, consistent with isolated tissue studies.
    • These findings refine methodologies for evaluating opioid drug efficacy and safety.