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Related Experiment Videos

The temperature-sensitive defect in polyoma virus P155 mutant.

C Galup, E Herring-Gillam, B S Sylla

    Virus Research
    |September 1, 1984
    PubMed
    Summary

    A temperature-sensitive polyoma virus mutant, P155, shows that large T antigen has distinct roles in viral replication and cell transformation. This finding helps understand polyoma virus functions.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Cellular Transformation

    Background:

    • Polyoma virus P155 is a temperature-sensitive mutant.
    • It transforms cells normally but replicates poorly under restrictive conditions.

    Purpose of the Study:

    • To investigate the function of large T antigen in polyoma virus replication and cell transformation.
    • To map the temperature-sensitive mutation in P155.

    Main Methods:

    • Genetic mapping of the temperature-sensitive mutation in P155.
    • Analysis of viral DNA and large T antigen thermolability.

    Main Results:

    • The temperature-sensitive character of P155 maps to the large T antigen gene.
    • Large T antigen is thermolabile in P155-infected cells.

    Conclusions:

    • Large T antigen plays distinct roles in viral replication versus cell transformation.
    • The P155 phenotype suggests differential functions for large T antigen.

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