Establishment of human glioblastoma multiforme cell line, G5A

A new cell line, G5A, was established from a human glioblastoma multiforme of the primary site and maintained for 25 months, subcultivated 36 times. The cloned cells showed morphologically epithelial-like patterns and loss of glial filaments. The cells inoculated in the nude mice produced tumors in 100% of the mice. The plating efficiency of the cell line was 20.9% in a standard culture. The modal chromosome number was 63 with many morphological abnormalities. The generation time was 22.9 hours and the parameters of the cell cycle were clarified by the percent labeled mitosis analysis. No morphological differentiation of the cell line was induced by the chemical differentiation-inducers.