Release of multivesicular bodies from platelets as observed in SEM

Scanning EM was used to study platelet shape changes induced by collagen in citrated PRP. While using a modified fixation technique known to protect phospholipids from extraction during alcohol dehydration a release of multivesicular bodies from platelets was observed. Maximum frequency of their appearance correlated with the onset of a release reaction from platelets as seen in an aggregometer curve. Depicted structures may thus be related to the release of different platelet constituents.