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Role of carbohydrates in cell membrane receptors for vesicular stomatitis virus

L Seganti, F Superti, P Mastromarino

    Bollettino Dell'Istituto Sieroterapico Milanese
    |January 1, 1982
    PubMed

    Insights

    Goose red blood cell and BHK 21 cell membrane receptors are crucial for vesicular stomatitis virus (VSV) attachment. Removing key sugars like mannose, galactose, and fucose significantly reduces VSV binding to these receptors.

    Area of Science:

    • Virology
    • Cell Biology
    • Biochemistry

    Background:

    • Vesicular stomatitis virus (VSV) utilizes cell surface receptors for entry.
    • Carbohydrate structures on cell membranes are implicated as potential viral receptors.

    Purpose of the Study:

    • To investigate the role of specific carbohydrate components in goose red blood cell and BHK 21 cell membranes as receptors for VSV.
    • To determine the impact of enzymatic removal of cell surface carbohydrates on VSV attachment.

    Main Methods:

    • Enzymatic modification of intact goose red blood cell and BHK 21 cell membranes.
    • Assessing the interaction between VSV and treated cells to quantify viral attachment.

    Main Results:

    • Removal of mannose from cell membranes decreased VSV attachment.
    • Elimination of galactose and fucose also significantly reduced VSV binding to cell receptors.

    Conclusions:

    • Mannose, galactose, and fucose are important carbohydrate components of cell membrane receptors for VSV.
    • These specific sugars play a critical role in the initial attachment of VSV to host cells.

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