Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

New developments in nucleic acid hybridization.

H Wolf, M Haus, U Leser

    IARC Scientific Publications
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Oral hairy leukoplakia in human immunodeficiency virus positive patients: clinical and etiopathogenic aspects].

    Acta otorrinolaringologica espanola·1992
    Same author

    Reactivities of HIV-1 gag-derived peptides with antibodies of HIV-1-infected and uninfected humans.

    AIDS research and human retroviruses·1992
    Same author

    Assessment of major histocompatibility complex class I interaction with Epstein-Barr virus and human immunodeficiency virus peptides by elevation of membrane H-2 and HLA in peptide loading-deficient cells.

    European journal of immunology·1992
    Same author

    Localization and identification of T-(Gal beta 1-3GalNAc alpha 1-O-R) and T-like antigens in experimental rat bladder cancer.

    The Journal of urology·1992
    Same author

    Human urinary bladder carcinoma glycoconjugates expressing T-(Gal beta(1-3)GalNAc alpha 1-O-R) and T-like antigens: a comparative study using peanut agglutinin and poly- and monoclonal antibodies.

    Cancer research·1992
    Same author

    Determination of placental ferritin (PLF)-positive lymphocytes in women in early stages of breast cancer.

    International journal of cancer·1992
    Same journal

    North America.

    IARC scientific publications·2016
    Same journal

    Central and South America.

    IARC scientific publications·2016
    Same journal

    Africa.

    IARC scientific publications·2016
    Same journal

    Data availability and presentation.

    IARC scientific publications·2016
    Same journal

    Age standardization.

    IARC scientific publications·2016
    Same journal

    Data processing.

    IARC scientific publications·2016
    See all related articles

    This study introduces novel, accessible hybridization probes for nucleic acid detection. A new indirect sandwich test enables versatile sequence specificity using a single labeled probe, simplifying laboratory workflows.

    Area of Science:

    • Molecular Biology
    • Biotechnology
    • Biochemistry

    Background:

    • Nucleic acid hybridization is a cornerstone technique in specialized research laboratories.
    • Existing methods often require specialized equipment and multiple probes for different sequences.

    Purpose of the Study:

    • To describe hybridization probes and techniques that can be prepared with less specialized equipment.
    • To introduce a novel indirect 'sandwich' hybridization test for versatile nucleic acid detection.
    • To evaluate the usability of fixed and embedded materials for in situ hybridization.

    Main Methods:

    • Development of a novel indirect 'sandwich' hybridization assay.
    • Utilization of a single, universally applicable labeled probe for various sequence specificities.

    Related Experiment Videos

  • Critical comparison of different labels and hybridization techniques.
  • Testing and evaluation of fixed and embedded materials for in situ hybridization.

    • Main Results:

    • The described hybridization probes can be prepared using less specialized laboratory equipment.
    • The new indirect 'sandwich' hybridization test allows for the use of a single labeled probe for diverse sequence detection.
    • The study provides a comparative analysis of various labels and hybridization methods.
    • The usability of fixed and embedded materials for in situ hybridization was assessed.

    Conclusions:

    • Accessible hybridization probe preparation is feasible with reduced equipment requirements.
    • The novel sandwich hybridization assay offers enhanced versatility and efficiency in nucleic acid detection.
    • The findings support broader application of hybridization techniques in various laboratory settings.