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Respiratory control in Micrococcus lysodeikticus.

M Rosenberg, I Friedberg

    Journal of Bioenergetics and Biomembranes
    |February 1, 1984
    PubMed
    Summary
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    Phosphate (Pi) significantly boosts Micrococcus lysodeikticus cell respiration, especially in Pi-deprived cells. This effect is linked to Pi uptake and polyphosphate formation, driving proton exchange and increasing respiration rates.

    Area of Science:

    • Microbiology
    • Cellular Respiration
    • Biochemistry

    Background:

    • Micrococcus lysodeikticus is a bacterium known for its metabolic versatility.
    • Cellular respiration is a fundamental process for energy production in microorganisms.
    • Phosphate (Pi) availability can influence bacterial metabolic pathways.

    Purpose of the Study:

    • To investigate the effect of phosphate (Pi) on the respiration rate of Micrococcus lysodeikticus.
    • To elucidate the mechanism by which Pi influences cellular respiration.
    • To determine the specificity and concentration dependence of Pi's effect on respiration.

    Main Methods:

    • Measuring respiration rates of Pi-deprived and Pi-grown Micrococcus lysodeikticus cells.
    • Assessing the impact of Pi and arsenate on respiration.

    Related Experiment Videos

  • Investigating the effects of succinate and valinomycin on respiration.
  • Analyzing the relationship between Pi concentration and respiration rate.

    • Main Results:

    • Phosphate (Pi) markedly increased the respiration rate of Pi-deprived cells, with the effect diminishing upon Pi consumption.
    • The stimulation of respiration by Pi was specific and concentration-dependent.
    • Pi-stimulated respiration involved rapid Pi uptake and polymerization to polyphosphate via ATP.
    • These processes were coupled to proton influx, leading to proton efflux and increased respiration.

    Conclusions:

    • Phosphate (Pi) acts as a key regulator of respiration in Micrococcus lysodeikticus.
    • The mechanism involves Pi uptake, polyphosphate synthesis, and proton motive force generation.
    • This highlights a specific metabolic control exerted by inorganic phosphate in bacteria.