Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Generating human monoclonal antibodies.

A M Neville, P A Edwards, M J O'Hare

    Medical Oncology and Tumor Pharmacotherapy
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Is follow-up CT imaging of the chest and abdomen necessary after preoperative neoadjuvant therapy in rectal cancer patients without evidence of metastatic disease at diagnosis?

    Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2013
    Same author

    Contrast-enhanced free-breathing 3D T1-weighted gradient-echo sequence for hepatobiliary MRI in patients with breath-holding difficulties.

    European radiology·2013
    Same author

    Cancer-testis antigen expression in triple-negative breast cancer.

    Annals of oncology : official journal of the European Society for Medical Oncology·2010
    Same author

    The NRG1 gene is frequently silenced by methylation in breast cancers and is a strong candidate for the 8p tumour suppressor gene.

    Oncogene·2009
    Same author

    Hyperproliferation of PKD1 cystic cells is induced by insulin-like growth factor-1 activation of the Ras/Raf signalling system.

    Kidney international·2007
    Same author

    Identification of transmembrane proteins as potential prognostic markers and therapeutic targets in breast cancer by a screen for signal sequence encoding transcripts.

    The Journal of pathology·2006
    Same journal

    Treatment of ovarian cancer: the state of the art.

    Medical oncology and tumor pharmacotherapy·1993
    Same journal

    Clinical evaluation of the CORDIS vascular access port systems: a multicenter study.

    Medical oncology and tumor pharmacotherapy·1993
    Same journal

    Expression of an adhesion molecule and homing in B-cell chronic lymphocytic leukaemia: I. Application of the HEV-binding assay to a clinical series.

    Medical oncology and tumor pharmacotherapy·1993
    Same journal

    Ultrastructural investigation of DNA in megakaryoblastic leukemia by using osmium-ammine-B: comparison with several types of leukemia.

    Medical oncology and tumor pharmacotherapy·1993
    Same journal

    T-lymphocyte sub-populations in orbito-ocular granulocytic sarcoma (OOGS) and acute myelocytic leukemia (AML): a preliminary study.

    Medical oncology and tumor pharmacotherapy·1993
    Same journal

    Low dose cyclophosphamide, alpha-interferon and continuous infusions of interleukin-2 in advanced renal cell carcinoma.

    Medical oncology and tumor pharmacotherapy·1993
    See all related articles

    Generating human monoclonal antibodies for tumor cell surface antigens using hybridoma methods is challenging. In vitro immunostimulation techniques may be necessary for reliable production of these crucial reagents.

    Area of Science:

    • Immunology
    • Biotechnology
    • Oncology

    Background:

    • Human monoclonal antibodies are vital tools in cancer research and therapy.
    • Current methods for generating human monoclonal antibodies, particularly against tumor antigens, face limitations.

    Purpose of the Study:

    • To review existing methods for human monoclonal antibody generation.
    • To assess the feasibility of producing human monoclonal antibodies against human tumor cell surface antigens.
    • To identify potential strategies for overcoming production challenges.

    Main Methods:

    • Review of current literature on hybridoma technology and antibody generation.
    • Analysis of the specificities and limitations of available reagents.
    • Discussion of in vitro immunostimulation techniques as alternative or complementary approaches.

    Related Experiment Videos

    Main Results:

    • Hybridoma methods rarely yield human monoclonal antibodies targeting human tumor cell surface antigens.
    • The specificities of currently available reagents for this purpose are limited.
    • In vitro immunostimulation presents a promising avenue for reliable antibody production.

    Conclusions:

    • Reliable production of human monoclonal antibodies against tumor antigens requires advanced techniques.
    • In vitro immunostimulation methods show potential for overcoming the limitations of traditional hybridoma approaches.
    • Further research into optimizing in vitro immunostimulation is warranted for clinical applications.