Lymphoblast transformation studies in sarcoidosis

Tissue culture studies of lymphnode lymphocytes, obtained from Sarcoidosis patients revealed lymphoblast transformation (morphologically observed) after incubation with Kveim antigen. Simultaneously we could obtain peripherical blood-lymphocytes from some patients. Lymphocytes from both compartments showed a positive correlated blasttransformation. Spontaneous blasttransformation was observed in cultures supplemented with fetal calf serum, however, this phenomenon was not observed in cultures with human AB serum. The results, together with the discrepance of spontaneous lymphoblast transformation will be discussed.