Studies on protein production by yeasts. IV. Incremental feeding modulus

Area of Science:

• Biotechnology
• Microbial Fermentation
• Biochemical Engineering

Background:

• Saccharomyces cerevisiae is a key microorganism for industrial protein production.
• Optimizing fermentation conditions is crucial for maximizing yield and economic viability.
• Strain Gr. 104 demonstrated high efficiency in preliminary protein production assessments.

Purpose of the Study:

• To identify the optimal incremental feeding modulus for Saccharomyces cerevisiae Gr. 104.
• To achieve the highest economical yield in protein production.
• To investigate the relationship between feeding rates and yeast biomass production.

Main Methods:

• Utilized a 5-liter laboratory fermentor for experiments.
• Employed molasses, ammonium sulphate, and potassium dihydrogen phosphate as substrates.
• Controlled pH, temperature, and aeration to optimal levels.
• Tested incremental feeding rates ranging from 1.14 to 1.22.

Main Results:

• The optimal incremental feeding modulus for S. cerevisiae Gr. 104 was determined to be 1.18.
• This rate corresponds to the molasses feeding rate during exponential growth.
• The highest economical yeast yield was achieved at the optimal feeding rate.
• Substrate utilization was maximized, with yields approaching theoretical calculations.

Conclusions:

• An incremental feeding modulus of 1.18 is optimal for maximizing economical protein yield in Saccharomyces cerevisiae Gr. 104.
• Controlled fermentation with optimized feeding strategies enhances substrate efficiency.
• This finding contributes to more cost-effective industrial yeast production.