Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyamines as physiological substrates for transglutaminases.

J E Folk, M H Park, S I Chung

    The Journal of Biological Chemistry
    |April 25, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Treatment outcomes of patients with Atopic Dermatitis (AD) treated with dupilumab through the Early Access to Medicines Scheme (EAMS) in the UK.

    The Ulster medical journal·2021
    Same author

    Possible transmission of Laugier-Hunziker syndrome by allogeneic peripheral blood stem cell transplantation.

    Clinical and experimental dermatology·2020
    Same author

    Frontal fibrosing alopecia: a descriptive cross-sectional study of 711 cases in female patients from the UK.

    The British journal of dermatology·2020
    Same author

    Presence of the HLA-A*3101 allele in a familial case of drug reaction with eosinophilia and systemic symptoms, secondary to carbamazepine.

    Clinical and experimental dermatology·2014
    Same author

    Scombrotoxin poisoning: an important differential diagnosis for anaphylaxis.

    Acute medicine·2011
    Same author

    The Positive Signs of Neurosis.

    Canadian Medical Association journal·2010
    Same journal

    Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

    The Journal of biological chemistry·2026
    Same journal

    Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

    The Journal of biological chemistry·2026
    Same journal

    Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

    The Journal of biological chemistry·2026
    Same journal

    YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

    The Journal of biological chemistry·2026
    Same journal

    ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

    The Journal of biological chemistry·2026
    Same journal

    PAX8 controls proximal tubule epithelial identity and stress response through epigenetic modification of distal regulatory elements.

    The Journal of biological chemistry·2026
    See all related articles

    Polyamines like putrescine and spermidine are incorporated into proteins in human lymphocytes and rat seminal plasma. This suggests polyamines act as substrates for transglutaminases in both cellular and extracellular environments.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Background:

    • Polyamines are essential cations involved in numerous cellular processes.
    • Transglutaminases are enzymes that catalyze the formation of isopeptide bonds.

    Purpose of the Study:

    • To investigate the role of polyamines as substrates for transglutaminases.
    • To compare polyamine incorporation into proteins in cellular and extracellular systems.

    Main Methods:

    • Treatment of human blood lymphocytes with [3H]putrescine.
    • Enzyme-mediated clotting of rat seminal plasma with 14C-labeled spermidine and spermine.
    • Analysis of proteolytic digests of protein fractions using mass spectrometry.

    Main Results:

    Related Experiment Videos

    • Polyamines were incorporated into specific proteins in lymphocytes.
    • Labeled polyamine derivatives, including N-(gamma-glutamyl)putrescine and N1-(gamma-glutamyl)spermidine, were identified in cellular digests.
    • Polyamines were incorporated into seminal plasma proteins during clotting.
    • Multiple labeled polyamine derivatives, including bis(gamma-glutamyl) derivatives, were identified in extracellular digests, suggesting cross-linking.

    Conclusions:

    • Polyamines serve as substrates for transglutaminases in both cellular and extracellular settings.
    • Distinct substrate properties of polyamines exist between cellular and extracellular environments.
    • Evidence suggests polyamine-mediated cross-linking of proteins occurs in extracellular fluids, but not in cells.