Certain immunological relationships between the pancreatic kallikrein and spermatozoa

The immunological relationship between kallikrein and acrosomal extracts from bull, ram, boar, and human spermatozoa was studied. Highly purified porcine pancreatic kallikrein was used and rabbit antikallikrein serum was produced. The immunochemical analysis did not reveal any antigenic relationship between kallikrein and the acrosomal extracts from spermatozoa of these species. Preincubation of spermatozoa in rabbit antikallikrein serum did not induce any changes in the acrosomal proteolytic activity. The antikallikrein serum significantly inhibited the motility of bull and boar spermatozoa but did not exert any marked influence on the motility of ram and human spermatozoa.