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Related Experiment Videos

Cytotoxic leukocyte reaction.

E Holopainen, T Palva, P Stenberg

    Acta Oto-Laryngologica
    |March 1, 1980
    PubMed
    Summary

    The cytotoxic leukocyte test shows moderate reproducibility and is influenced by serum factors. Heating serum, EDTA, DSCG, antihistamines, and cortisone significantly alter test results, impacting its reliability for food antigen testing.

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    Area of Science:

    • Immunology
    • Allergy Testing
    • In Vitro Diagnostics

    Background:

    • The cytotoxic leukocyte test is utilized for identifying hypersensitivity to various substances, including food antigens.
    • Assessing the reliability and influencing factors of this diagnostic method is crucial for accurate clinical application.

    Purpose of the Study:

    • To evaluate the reproducibility of the cytotoxic leukocyte test.
    • To investigate the impact of serum factors and specific inhibitors on the test's reactivity to food antigens.

    Main Methods:

    • Cytotoxic leukocyte tests were performed on blood samples from multiple subjects.
    • Tests were repeated to assess day-to-day variations.
    • In vitro experiments involved manipulating reactive serum and cells, and applying inhibitors like EDTA, DSCG, antihistamine, and cortisone.

    Main Results:

    • The test demonstrated 78% agreement between technicians, with statistical error at 0.46.
    • Reproducibility over three days showed 18-20% deviation.
    • Positive reactions were transferable via serum, but heat inactivation abolished reactivity in most cases.
    • EDTA consistently blocked reactions, while DSCG, antihistamine, and cortisone showed variable inhibition.

    Conclusions:

    • The cytotoxic leukocyte test exhibits moderate reproducibility.
    • Test results are significantly influenced by heat-labile serum factors and can be modulated by EDTA, DSCG, antihistamine, and cortisone.
    • Further research is needed to refine the methodology for reliable food antigen testing.

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