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[Bidimensional immunoelectrophoretic study of the antigenic composition of the membrane in various mycoplasma
Abstract:
Membrane antigenic composition of Acheloplasma laidlawii PG9, A. granularum BTS-39, and Mycoplasma fermentans PG 18(G) was determined by means of bidimensional immunoelectrophoresis in the presence of sodium desoxycholate 0.5%. Depending upon the mycoplasma species from which membranes were obtained, 7 to 15 antigens were evidenced. Using sodium desoxycholate presents the advantage over non-ionic detergents to dissolve better the mycoplasmic membrane antigenic complexes. A comparative study of five strains belonging to the above-noted species confirms the serological heterogeneity of the Mycoplasmateles order and shows variability at the membrane antigenic composition level of Acheloplasma laidlawii.
Insights
This study analyzed the membrane antigens of Acheloplasma laidlawii and Mycoplasma fermentans using bidimensional immunoelectrophoresis. Findings reveal significant antigenic variability within Acheloplasma laidlawii strains, highlighting serological heterogeneity in the Mycoplasmateles order.
Area of Science:
- Microbiology
- Immunology
- Molecular Biology
Context:
- The antigenic composition of mycoplasma cell membranes is crucial for understanding their biology and host interactions.
- Previous studies have utilized various detergents for membrane antigen analysis, with varying efficiencies.
Purpose:
- To determine and compare the membrane antigenic composition of specific Acheloplasma and Mycoplasma species.
- To evaluate the efficacy of sodium deoxycholate in solubilizing mycoplasmal membrane antigens for bidimensional immunoelectrophoresis.
Summary:
- Bidimensional immunoelectrophoresis in the presence of 0.5% sodium deoxycholate was employed to analyze membrane antigens from Acheloplasma laidlawii, A. granularum, and Mycoplasma fermentans.
- Between 7 to 15 distinct antigens were detected per species, with sodium deoxycholate proving superior to non-ionic detergents in dissolving antigenic complexes.
- Comparative analysis of five strains confirmed serological heterogeneity within the Mycoplasmateles order and demonstrated antigenic variability in Acheloplasma laidlawii.
Impact:
- Provides a detailed antigenic profile of key mycoplasma species, aiding in their classification and identification.
- Highlights the utility of sodium deoxycholate in mycoplasma membrane antigen analysis.
- Contributes to understanding the serological diversity within the Mycoplasmateles order, particularly the variability in Acheloplasma laidlawii membrane composition.