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A highly efficient method of cell infection with encephalomyocarditis virus RNA
Acta Virologica
|June 1, 1980
Abstract:
A method for infecting Krebs-II cells with encephalomyocarditis virus RNA based on treatment of the cells with DEAE-dextran at high concentration was elaborated. The specific infectivity of virion RNA was 3 X 10(8) plaque-forming units per microgram, i. e. comparable to the specific infectivity of the whole virus. A high proportion of the cell population could be infected by this method.