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Related Experiment Videos

Factor VIII collection by pheresis.

B C McLeod, R J Sassetti, E R Cole

    Lancet (London, England)
    |September 27, 1980
    PubMed
    Summary
    This summary is machine-generated.

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    This study introduces a novel blood donation method for isolating cryoprecipitate proteins. The procedure, involving sequential plasma exchanges, efficiently yields substantial Factor VIII, potentially reducing hepatitis risk for recipients.

    Area of Science:

    • Hematology
    • Transfusion Medicine
    • Biochemistry

    Background:

    • Cryoprecipitate is a blood component rich in specific proteins like Factor VIII.
    • Current methods for cryoprecipitate collection can be inefficient and carry risks.
    • There is a need for improved procedures to maximize protein yield and minimize donor exposure.

    Purpose of the Study:

    • To develop and evaluate a new blood donation procedure for selectively obtaining cryoprecipitate proteins.
    • To assess the safety and efficiency of sequential automated plasma exchange for cryoprecipitate supernatant collection.
    • To determine the yield of Factor VIII using this novel method.

    Main Methods:

    • Sequential automated plasma exchange was used, replacing donor plasma with autologous cryoprecipitated supernatant.

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  • Fresh plasma was processed into cryoprecipitate and supernatant, both frozen.
  • Six donors underwent 26 exchange donations (1.5-2 litres each).

    • Main Results:

    • No adverse effects were observed in donors during the procedure.
    • Factor VIII yield per unit decreased during donation but remained substantial.
    • Average total Factor VIII yield per donation was 730 U.

    Conclusions:

    • The new procedure is safe and effective for obtaining cryoprecipitate proteins.
    • This method increases Factor VIII yield per donor, potentially reducing hepatitis risk.
    • Optimized cryoprecipitate collection can improve therapeutic protein availability.