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Related Experiment Videos

Membrane associated enzyme complexes containing lambda type immunoglobulin A.

K Sudo, T Kanno

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |October 23, 1980
    PubMed
    Summary

    Membrane-associated enzyme complexes containing alkaline phosphatase, arylamidase, and L-gamma-glutamyltranspeptidase exhibit heterogeneity. These complexes are linked to immunoglobulin A, showing specific alpha heavy chain and lambda light chain types.

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    Area of Science:

    • Biochemistry
    • Immunology
    • Cell Biology

    Background:

    • Membrane-associated enzyme complexes play crucial roles in cellular functions.
    • Understanding the composition and properties of these complexes is vital for elucidating biological mechanisms.

    Purpose of the Study:

    • To investigate the properties of membrane-associated enzyme complexes.
    • To determine the association of immunoglobulin A with these enzyme complexes.
    • To analyze the specificity of immunoglobulin binding.

    Main Methods:

    • Gel filtration using Sephadex G-200 to exclude enzyme complexes.
    • Electrophoresis to determine electrophoretic mobility.
    • Density gradient ultracentrifugation to assess density heterogeneity.

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  • Immunological fixation using specific antibodies (anti-IgA, anti-light chain lambda).

    • Main Results:

    • Enzyme complexes of alkaline phosphatase, arylamidase, and L-gamma-glutamyltranspeptidase were characterized.
    • Electrophoretic mobility was observed in the alpha-1 portion.
    • Density heterogeneity was identified, with enzyme activities present in both high and low-density particles.
    • Immunoglobulin A, specifically alpha heavy chains and lambda light chains, was found to be associated with high-density enzyme complexes.
    • Non-specific binding was ruled out due to observed isotype specificity.

    Conclusions:

    • The heterogeneity in density of membrane-associated enzyme complexes is attributed to the presence of immunoglobulin A.
    • The immunoglobulin A associated with these complexes exhibits specific isotype characteristics (alpha heavy chains, lambda light chains).
    • This isotype specificity suggests a non-random association and has implications for understanding circulating autoantibodies.