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Related Experiment Videos

Enkephalinases.

C Gorenstein, S H Snyder

    Proceedings of the Royal Society of London. Series B, Biological Sciences
    |October 29, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Researchers identified key brain peptidases, including novel enkephalinase B, involved in inactivating enkephalins. Enkephalinase A1 activity correlates with opiate receptors, suggesting a role in pain modulation.

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    Area of Science:

    • Neuroscience
    • Biochemistry
    • Enzymology

    Background:

    • Enkephalins are endogenous opioid peptides crucial for pain regulation.
    • Their physiological inactivation is mediated by various peptidases.
    • Identifying these enzymes is key to understanding enkephalin signaling.

    Purpose of the Study:

    • To characterize membrane-associated brain peptidases responsible for enkephalin inactivation.
    • To differentiate these peptidases from other enzymes like angiotensin converting enzyme.
    • To investigate the distribution and properties of identified enkephalin-degrading enzymes.

    Main Methods:

    • Enzyme activity assays using brain tissue preparations.
    • Detergent solubilization of membrane-bound peptidases.

    Related Experiment Videos

  • DEAE column chromatography for enzyme separation and purification.
  • Purification of enkephalinase A1 to homogeneity.

    • Main Results:

    • Two carboxyl-directed dipeptidylpeptidases (enkephalinase A1 and A2) were identified, producing Tyr-Gly-Gly.
    • A novel amino-terminal-directed dipeptidylpeptidase (enkephalinase B) generating Tyr-Gly was discovered.
    • Enkephalinase A1 activity showed regional similarity to opiate receptor distribution.
    • All identified peptidases were solubilized and separated using chromatography.

    Conclusions:

    • Specific brain peptidases, including novel enkephalinase B, play a role in enkephalin inactivation.
    • Enkephalinase A1's distribution suggests a functional link to opioid signaling pathways.
    • These findings provide insights into the regulation of enkephalinergic neurotransmission.