Immunofluorescent localization of gamma-glutamyl transferase in rat and bovine tissues

A distribution of the enzyme in tissue sections was studied by direct fluorescent technique using immunoglobulins from rabbits immunized with the rat and bovine kidney enzyme. For studies rat and bovine sections of kidneys, livers, pancreas, and small intestine were used. Cell distribution of the enzyme by immunofluorescent method was the same as that by the classical histochemical method. No differences in the enzyme distribution were noted when either immunoglobulins anti-heavy form or immunoglobulins anti light form of the enzyme were used. Bovine kidney enzyme coupled to diazotized p-aminobenzoyl-hexamethylenediamine-sepharose is a effective sorbant for affinity chromatography of antibody. Precipitates formed between antibodies and purified rat or bovine gamma-glutamyl transferases preserves over 60% of the initial enzyme activity.