Protein biosynthesis by rat gastric mucosal cells in suspension

Suspensions of cells freshly isolated from rat gastric mucosa by the pronase technique did not synthesize protein unless washed. Synthetic activity proceeded for at least 3h in washed cells, but unless three or four washing cycles were given the synthesized protein was subsequently degraded. Supplementation of the incubation medium with 3% w/v bovine serum albumin markedly increased synthetic activity. Special consideration was given to the control of artifacts caused by bacterial activity, oxygen deficiency and adsorption of radioleucine.