Conversion of 14C-labeled eicosapentaenoic acid (n-3) to leukotriene C5

14C-labeled eicosapentaenoic acid (n-3) was converted by mouse mastocytoma cells to 5-hydroxy-6-S-glutathionyl-7,9,11,14,17-eicosapentaenoic acid (leukotriene C5). The identification was based on comparisons with previously characterized unlabeled material by high-performance liquid chromatography, ultraviolet spectroscopy, and conversion by gamma-glutamyl transpeptidase to leukotriene D5.