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Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction
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Published on: April 26, 2011

High-performance liquid chromatography to measure lecithin/sphingomyelin ratio

H T Ng, C Y Chen, R D Wei

    International Journal of Gynaecology and Obstetrics: the Official Organ of the International Federation of Gynaecology and Obstetrics
    |January 1, 1980
    PubMed

    The amniotic fluid lecithin/sphingomyelin (L/S) ratio was measured by high-performance liquid chromatography in 85 pregnant women of varying gestational ages to determine fetal lung maturity. Respiratory distress syndrome occurred in 14 of the 15 infants whose L/S ratio before birth was lower than 3.0. High-performance liquid chromatography for measurement of the amniotic fluid L/S ratio provides a sensitive index of the functional development of the fetal lung and therefore of the risk of the neonate developing respiratory distress syndrome.

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