Specific and sensitive method for the determination of tobanum using gas chromatography with electron-capture and...

Quantitative gas--liquid chromatographic (GLC) and GLC--mass spectrometric (MS) methods for the determination of Tobanum, a new beta-blocking agent, in human plasma have been developed. After solvent extraction, a bis-trifluoroacetyl derivative is formed which is measured by an electron-capture detector. The quantitation is controlled by using an internal standard, propranolol hydrochloride, which is added to all samples. The electron-capture detector response is linear in the concentration range used, 5--150 ng/ml. The identity and quantity of the GLC peaks is confirmed by GLC--MS. The minimum detectable concentration of Tobanum is 1 ng using 3-ml plasma samples.