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Related Experiment Videos

Somatostatin binding factor from chicken pancreas.

F Diel, N Bethge, E Schneider

    Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
    |February 1, 1981
    PubMed
    Summary

    Researchers identified a soluble somatostatin binding factor in chicken pancreas extracts. This factor, with an estimated molecular weight of 40,000, shows specific binding characteristics and is concentrated in cytosol fractions.

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    Area of Science:

    • Endocrinology
    • Molecular Biology
    • Biochemistry

    Background:

    • Somatostatin plays a crucial role in regulating various physiological processes.
    • Understanding somatostatin binding mechanisms is key to deciphering its functions.
    • Chicken pancreas is a potential source for novel binding factors.

    Purpose of the Study:

    • To detect and characterize a soluble somatostatin binding factor in chicken pancreas.
    • To determine the binding properties and molecular characteristics of this factor.

    Main Methods:

    • Radio-labeling of Tyr1-somatostatin with 125I.
    • Binding assays using cell-free chicken pancreas extracts.
    • Gel filtration and polyacrylamide disc gel electrophoresis for molecular weight and mobility determination.

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    Main Results:

    • A soluble somatostatin binding factor was detected in chicken pancreas cytosol.
    • The binding molecule has an estimated molecular weight of 40,000 (Mr).
    • Specific binding was inhibited by cold somatostatin, Ca2+, and reduced thiol-reagents, with varying cross-reactivity to other hormones.

    Conclusions:

    • A novel soluble somatostatin binding factor exists in chicken pancreas.
    • This factor exhibits specific binding properties and a distinct molecular weight.
    • The splenic lobe area of the chicken pancreas showed significantly higher binding activity.